DR. RAMESHA A

Name: Dr. Ramesha A

Designation: Assistant Professor

Qualification: M.Sc., PhD., KSET

Year of Joining: 2020

Work Experience: Teaching - 6 years & Industrial - 2 years

Subject Specialization: Microbiology

RESEARCH

characterization and evaluation of biological activities of Oosporein were carried out from Cochliobolus kusanoi an endophytic fungus of Nerium oleander L. which exhibited antimicrobial and cytotoxic activities. The cytotoxic effects of oosporein on MDCK and RAW264.7 cells is mediated through ROS induced oxidative stress leading to increase in lipid peroxidation, and elevated levels of GSH results to loss in MMP and DNA damage at the cellular level. These events were supported by the q-RT-PCR quantification of the oxidative and apoptotic biomarker gene expression. The high prevalence of these fungi in temperate climates further warrants the need of safe food grain storage and processing practices to control the toxic effects of oosporein to humans and live stock.

PUBLICATIONS (PAPER, BOOKS, ARTICLES ETC)

Research articles Published in Journals

Priyanka V., Ramesha A., Devaraja Gayathri., Vasudha M. (2021). Molecular characterization of non-biogenic amines producing Lactobacillus plantarum GP11 isolated from traditional pickles using HRESI-MS analysis. Journal food science and technology, 58(6):2216-2226. (Impact factor; 3.117)
Shobha Balagangadhara, Thimappa Ramachandrappa Lakshmeesha, Mohammad Azam Ansari, Ahmad Almatroudi, Mohammad A Alzohairy, Sumanth Basavaraju, Ramesha Alurappa, Siddapura Ramachandrappa Niranjana, Srinivas Chowdappa (2020). Mycosynthesis of ZnO nanoparticles using Trichoderma spp isolated from rhizosphere soils and its synergistic antibacterial effect against Xanthomonas oryzae pv. oryzae. Journal of Fungi. 6:181 (Impact factor; 5.724)
Tanvi, D.A., Pratam, K.M., Lohit, R.T., Vijayalakshmi, B. K., Devaraja, T. N., Vasudha, M., Ramesh A., Prashantkumar, S.C., Gayathri, D (2020). Biosorption of heavy metal arsenic from Industrial Sewage of Davangere District, Karnataka, India, using indigenous fungal isolates. SN Applied Sciences, 2; 1860. https://doi.org/10.1007/s42452-020-03622-0
Devaraja Gayathri and Alurappa Ramesha, (2020). Gluten?hydrolyzing probiotics: An emerging therapy for patients with celiac disease (Review). World Academy of Sciences Journal, 2: 14.https://doi.org/10.3892/wasj.2020.55
Shubha J., Narasimha M.K., Ramesha A., Srinivas C. (2019). Production, Characterization of Indole Acetic Acid and its Bioactive Potential from Endophytic Fungi of Cymbidium aloifolium L. Journal of Biologically Active Products from Nature, Vol. 9 (5) 2019 pp 387- 409. (Impact factor: 1.21)
Ramesha alurappa and Srinivas chowdappa, (2018). Antimicrobial activity and phytochemical analysis of endophytic fungi isolated from ethno pharmaceutical plant Rauwolfia tetraphylla L. Journal of Pure and Applied Microbiology, Vol. 12(1), p. 317- 332. (Impact factor: 0.157)
Ramesha, A., Venkataramana, M., Nirmaladevi, D.,Srinivas, C., Gupta, V.K., Chandranayaka, S., (2015). Cytotoxic effects of Oosporein isolated from endophytic fungus Cochliobolus kusanoi. Frontiers in Microbiology, 6:870. (Impact factor: 6.064)
Nirmaladevi, D., Ramesha, A., Sunitha, V. H., Babu, R.L., Srinivas, C. (2015) Antimicrobial and cytotoxic effects of the bioactive pigment Bikaverin produced by fusarium oxysporum f. sp. lycopersici. Journal of Mycology and Plant Pathology, 45(1): 13-21.( NAAS Rate: 5.79)
Ramesha Alurappa, Madhusudhan Reddy Muthukurpalya Bojegowda, Vijith Kumar, Naveen kumar Mallesh and Srinivas Chowdappa, (2014). Characterization and bioactivity of Oosporein produced by Endophytic fungus Cochliobolus kusanoi isolated from Nerium oleander L. Natural product research. 28(23): 2217–2220. (Impact factor: 2.488)
Ramesha Alurappa, Madhusudhan Reddy Muthukurpalya Bojegowda, Vijith Kumar, Naveen kumar Mallesh and Srinivas Chowdappa, (2014). Characterization and bioactivity of Oosporein produced by Endophytic fungus Cochliobolus kusanoi isolated from Nerium oleander L. Natural product research. 28(23): 2217–2220. (Impact factor: 2.488)
Ramesha, A. and C. Srinivas, (2014) Antimicrobial activity and phytochemical analysis of crude extracts of endophytic fungi isolated from Plumeria acuminata L. and Plumeria obtusifolia L. European Journal of Experimental Biology, 4(2): 35-43.
Ramesha, A., Sunitha, V. H, and C. Srinivas, (2013) Antimicrobial activity of secondary metabolites from endophytic fungi isolated from Nerium oleander L. International Journal of Pharma and Bio Sciences, 4(1): 683 – 693.
Sunitha, V. H., Ramesha, A., Savitha, J., Srinivas, C., (2012). Amylase production by endophytic fungi Cylindrocephalum sp. Isolated from medicinal plant Alpinia calcarata (haw.) ROSCOE. Brazilian Journal of Microbiology, 1213-1221. (Impact factor: 2.214)
Hegde, S.V., Ramesha, A. and Srinvas, C. (2011). Optimization of amylase production from an endophytic fungi Discosia sp. isolated from Calophyllum inophyllum. Journal of Agricultural Technology, 7(3): 805-813. (Impact factor: 0.43)

Patent Filed

Rameha A and Srinivas C. Method for Production and Characterization of Oosporein from Endophytic fungus (Cochliobolus kusanoi).

Book Chapters Published

RameshaAlurappa, Srinivas Chowdappa, Radhakrishnan Narayanaswamy, Uma Rani Sinniah, Sudipta Kumar Mohanty and Mallappa Kumara Swamy (2017). Endophytic fungi and bioactive metabolites production: An update. Page no. 467- 494, Volume no. 2, Microbial Biotechnology, Springer Nature Singapore Pte Ltd. J.K. Patra et al. (eds.), https://doi.org/10.1007/978-981-10-7140-9_21. 2.
Ramesha A., Nirmala Devi. D, Sunitha V Hegde, Srinivas C. (2020) Biotechnological approaches for the enhancement of secondary metabolite production from endophytic fungi. Page no.135-155. Recent Advances in Application of Fungi and Fungal Metabolites: Applications in Healthcare New and Future Developments in Microbial Biotechnology and Bioengineering, Elsevier. https://doi.org/10.1016/B978-0-12-821006-2.00011-X

ITS rDNA Sequences deposited in Genbank

Alurappa, R., 2013. Cochliobolus kusanoi isolate (NOS-6) partial ITS rDNA Sequences (516 bp) deposited in the GenBank (NCBI). Accession number KF193636.
Ramesha, A. and Srinivas, C., 2014. Colletotrichum gloeosporioides strain (PAS1) partial ITS rDNA Sequences (518 bp) deposited in the GenBank (NCBI). Accession number KF864554.
Ramesha, A. and Srinivas, C., 2014. Fusarium oxysporum strain (POS3) partial ITS rDNA Sequences (486 bp) deposited in the Gen Bank (NCBI). Accession number KF864555.
Ramesha, A. and Srinivas, C., 2014. Curvularia sp. strain (RTFs6) partial ITS rDNA Sequences (532bp) deposited in the Gen Bank (NCBI). Accession number KF864556.
Alurappa, R. (2021). Aspergillus flavus isolate PBS5 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. Accession Number; MZ566566.1.
Alurappa, R. (2021). Aspergillus niger isolate GS2 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. Accession Number; MZ617325.1

Deposition of Crystal Data at CCDC

CCDC (Cambridge Crystallographic Data Centre) Citation – Ramesha Alurappa, Madhusudhan Reddy, Muthukurpalya Bojegowda, Vijith Kumar, Naveen Kumar Mallesh, Srinivas Chowdappa, CCDC 942418: Experimental Crystal Structure Determination, 2014, DOI: 10.5517/cc10mnlw.

NATIONAL AND INTERNATIONAL SEMINARS, WORKSHOPS AND CONFERENECES

Research Paper presented in Conferences:

Paper presentation on “Nephrotoxic effects of oosporein isolated from endophytic fungus Cochliobolus kusanoi” National conference on secondary metabolites of endophytic fungi/medicinal plants and their anticancer properties, Dept of Microbiology and Biotechnology, Bangalore University, 5th and 6th March 2015, Bangalore, Karnataka, India.
Oral presentation on “Characterization and bioactivity of oosporein produced by endophytic fungus Cochliobolus kusanoi isolated from Nerium oleander.” National seminar on Applications of Biotechnology in Human welfare, 28- 29th March 2014. Department of Studies in Microbiology, Davangere University, Davangere, Karnataka, India.
Oral presentation on “Characterization of antimicrobial pigment from endophytic fungus Cochliobolus kusanoi isolated from Nerium oleander” Seminar on Recent Trends In Microbial biotechnology, 28-29th May 2012, Department of Microbiology and Biotechnology, Bangalore University, Bangalore, Karnataka, India.
Poster presentation on “Antimicrobial activity of endophytic fungi isolated from medicinal plants of Apocynaceae” National conference on Environmental Sciences and Health hazards and 14th Annual Meeting of society for Science and Enviroment, 19-21st November 2012, Department of Microbiology and Biotechnology, Karnatak University, Dharwad, Karnataka, India.
Poster presentation on “Optimization of amylase production by endophytic fungi Cylindrocephalum sp. isolated from Alpinia calcarata”, National Conference on Recent advances in Mycological Research, 8-9th February, 2011, Centre for advanced studies in Botany, University of Madras, Chennai, India
Poster presentation on “Optimization of amylase production by endophytic fungi Discosia sp. isolated from Calophyllum inophyllum” National seminar on “Biodiversity: for Human welfare”, 7-8th April 2010, Department of studies and research in Microbiology, Mangalore University, Madikeri, Karnataka, India.
Poster Presentation on “Climate Change” organised by Dept. of Microbiology and Biotechnology, Bangalore University in association with Kanrnatatka Rajya Vignana Parishath on 12th March 2010, Dept. of Microbiology and Biotechnology, Bangalore University Bangalore, Karnataka, India.

Participation in Faculty Development Programmes:

Faculty development programme on " Essentials of Biomolecules: Nucleic acids and Peptides" Jan 2022-April 2022 (12 week course). Organized by NPTEL-AICTE, MHRD, Govt. of India.
One day Faculty Development Program on “Emotional intelligence” 20th October 2022 to 1st Jan 2022 (6 days). Organized by Department of Commerce, MSRCASC, Bangalore.
Faculty Development Program on “Different Framework for Holistic Education” 27th Dec 2021 to 1st Jan 2022 (6 days). Organized by swadeshi vijnana andolana, karnataka and MSRCASC, Bangalore.

Participation in Workshops:

Workshop on ‘Advanced experimental methodologies in biological research’ from 17-19th June 2021. Organized by Department of Biotechnology and Genetics, MSRCASC, Bangalore.
National Workshop on “Frontiers in Cell Biology and Biotechnology”28-29th December 2012. Organized by Department of Microbiology and Biotechnology, Bangalore University, Bangalore, Karnataka, India.
Workshop on “Use Statistical Softwares in Biological Research” 6-7th August 2011. Department of Microbiology and Biotechnology, Bangalore University, Bangalore, Karnataka, India.
Lecture Workshop on “Advances in Biological Sciences” 20-22nd January 2011. Poornaprajna Institute of Scientific Research, Bangalore, Karnataka, India.
Participated in DBT sponsored popular lecture workshop on the theme “Biotechnology in Health Care” 6th April 2011, conducted by Dept. of Microbiology and Biotechnology, Bangalore University, Bangalore, Karnataka, India.

Participation in Conferences:

National conference on secondary metabolites of endophytic fungi/medicinal plants and their anticancer properties, Dept of Microbiology and Biotechnology, Bangalore University, 5th and 6th March 2015, Bangalore, Karnataka, India.
National conference on Environmental Sciences and Health hazards and 14th Annual Meeting of society for Science and Environment, 19-21st November 2012,Department of Microbiology and Biotechnology, Karnatak University, Dharwad, Karnataka, India.
National Conference on Recent advances in Mycological Research, 8-9th February, 2011, Centre for advanced studies in Botany, University of Madras, Chennai, India
National Conference on Biotechnology and Society (NCBS-2012), 19th and 20th April 2012. Dept. of Biotechnology, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Karnataka, India.
National Conference on “Recent trends in Genomics and Proteomics” 26th and 27th May 2011, conducted by Dept. of Microbiology and Biotechnology, Bangalore University in association with Indian Academy Degree college. Dept. of Microbiology and Biotechnology, Bangalore University, Bangalore, Karnataka, India.